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sequencing ladder mix  (Thermo Fisher)


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    Thermo Fisher sequencing ladder mix
    Sequencing Ladder Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequencing+ladder+mix/DITHIOTHREITOL/10__3390_slash_agronomy13081990-66-72-102
    Average 99 stars, based on 1 article reviews
    sequencing ladder mix - by Bioz Stars, 2026-09
    99/100 stars

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    Sequencing:

    Article Title: Probing the RNA Structure of a Satellite RNA of Cucumber Mosaic Virus Using SHAPE Method
    Article Snippet: .. Sequencing reactions: [γ-32p]-ATP labeled primers (3.0 μL) and 0.5 × TE buffer (8.0 μL) were added to 1.0 μL of untreated satCMV TA-Tb RNA (1.0 μM, 2.0 μM or 10.0 μM corresponding to the SHAPE reaction), and primer-template solutions were incubated at 65 ◦C for 5 min and 35 ◦C for 5 min, followed by incubation on ice for 1 min. Primer extension was initiated by the addition of 8.0 μL of sequencing ladder mix (4.0 μL 5 × SSIII first strand buffer, 1.0 μL 0.1 M DTT, 1.0 μL 10 mM dNTP, 1.5 μL 10 mM ddTTP, 0.5 μL Superscript III (Invitrogen, Waltham, MA, USA); preheated at 52 ◦C for 1 min) and incubation at 52 ◦C in a thermostatic metal bath for 1 h. SHAPE reactions: [γ-32p]-ATP labeled primers (3.0 μL) were added to modified satCMV TA-Tb RNA (10.5 μL), and the primer-template solutions were incubated at 65 ◦C for 5 min and 35 ◦C for 5 min, followed by incubation on ice for 1 min. Primer extension was initiated by the addition of 6.5 μL SHAPE enzyme mix (4.0 μL 5 × SSIII First strand buffer, 1.0 μL 0.1 M DTT, 1.0 μL 10 mM dNTP, 0.5 μL Superscript III; preincubated at 52 ◦C for 1 min) and incubation at 52 °C for 1 h, resulting in the addition of 2′-O-adducts on the RNA template. ..

    Labeling:

    Article Title: Probing the RNA Structure of a Satellite RNA of Cucumber Mosaic Virus Using SHAPE Method
    Article Snippet: .. Sequencing reactions: [γ-32p]-ATP labeled primers (3.0 μL) and 0.5 × TE buffer (8.0 μL) were added to 1.0 μL of untreated satCMV TA-Tb RNA (1.0 μM, 2.0 μM or 10.0 μM corresponding to the SHAPE reaction), and primer-template solutions were incubated at 65 ◦C for 5 min and 35 ◦C for 5 min, followed by incubation on ice for 1 min. Primer extension was initiated by the addition of 8.0 μL of sequencing ladder mix (4.0 μL 5 × SSIII first strand buffer, 1.0 μL 0.1 M DTT, 1.0 μL 10 mM dNTP, 1.5 μL 10 mM ddTTP, 0.5 μL Superscript III (Invitrogen, Waltham, MA, USA); preheated at 52 ◦C for 1 min) and incubation at 52 ◦C in a thermostatic metal bath for 1 h. SHAPE reactions: [γ-32p]-ATP labeled primers (3.0 μL) were added to modified satCMV TA-Tb RNA (10.5 μL), and the primer-template solutions were incubated at 65 ◦C for 5 min and 35 ◦C for 5 min, followed by incubation on ice for 1 min. Primer extension was initiated by the addition of 6.5 μL SHAPE enzyme mix (4.0 μL 5 × SSIII First strand buffer, 1.0 μL 0.1 M DTT, 1.0 μL 10 mM dNTP, 0.5 μL Superscript III; preincubated at 52 ◦C for 1 min) and incubation at 52 °C for 1 h, resulting in the addition of 2′-O-adducts on the RNA template. ..

    Incubation:

    Article Title: Probing the RNA Structure of a Satellite RNA of Cucumber Mosaic Virus Using SHAPE Method
    Article Snippet: .. Sequencing reactions: [γ-32p]-ATP labeled primers (3.0 μL) and 0.5 × TE buffer (8.0 μL) were added to 1.0 μL of untreated satCMV TA-Tb RNA (1.0 μM, 2.0 μM or 10.0 μM corresponding to the SHAPE reaction), and primer-template solutions were incubated at 65 ◦C for 5 min and 35 ◦C for 5 min, followed by incubation on ice for 1 min. Primer extension was initiated by the addition of 8.0 μL of sequencing ladder mix (4.0 μL 5 × SSIII first strand buffer, 1.0 μL 0.1 M DTT, 1.0 μL 10 mM dNTP, 1.5 μL 10 mM ddTTP, 0.5 μL Superscript III (Invitrogen, Waltham, MA, USA); preheated at 52 ◦C for 1 min) and incubation at 52 ◦C in a thermostatic metal bath for 1 h. SHAPE reactions: [γ-32p]-ATP labeled primers (3.0 μL) were added to modified satCMV TA-Tb RNA (10.5 μL), and the primer-template solutions were incubated at 65 ◦C for 5 min and 35 ◦C for 5 min, followed by incubation on ice for 1 min. Primer extension was initiated by the addition of 6.5 μL SHAPE enzyme mix (4.0 μL 5 × SSIII First strand buffer, 1.0 μL 0.1 M DTT, 1.0 μL 10 mM dNTP, 0.5 μL Superscript III; preincubated at 52 ◦C for 1 min) and incubation at 52 °C for 1 h, resulting in the addition of 2′-O-adducts on the RNA template. ..

    Modification:

    Article Title: Probing the RNA Structure of a Satellite RNA of Cucumber Mosaic Virus Using SHAPE Method
    Article Snippet: .. Sequencing reactions: [γ-32p]-ATP labeled primers (3.0 μL) and 0.5 × TE buffer (8.0 μL) were added to 1.0 μL of untreated satCMV TA-Tb RNA (1.0 μM, 2.0 μM or 10.0 μM corresponding to the SHAPE reaction), and primer-template solutions were incubated at 65 ◦C for 5 min and 35 ◦C for 5 min, followed by incubation on ice for 1 min. Primer extension was initiated by the addition of 8.0 μL of sequencing ladder mix (4.0 μL 5 × SSIII first strand buffer, 1.0 μL 0.1 M DTT, 1.0 μL 10 mM dNTP, 1.5 μL 10 mM ddTTP, 0.5 μL Superscript III (Invitrogen, Waltham, MA, USA); preheated at 52 ◦C for 1 min) and incubation at 52 ◦C in a thermostatic metal bath for 1 h. SHAPE reactions: [γ-32p]-ATP labeled primers (3.0 μL) were added to modified satCMV TA-Tb RNA (10.5 μL), and the primer-template solutions were incubated at 65 ◦C for 5 min and 35 ◦C for 5 min, followed by incubation on ice for 1 min. Primer extension was initiated by the addition of 6.5 μL SHAPE enzyme mix (4.0 μL 5 × SSIII First strand buffer, 1.0 μL 0.1 M DTT, 1.0 μL 10 mM dNTP, 0.5 μL Superscript III; preincubated at 52 ◦C for 1 min) and incubation at 52 °C for 1 h, resulting in the addition of 2′-O-adducts on the RNA template. ..



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    sequencing ladder mix  (Thermo Fisher)
    99
    Thermo Fisher sequencing ladder mix
    Sequencing Ladder Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequencing+ladder+mix/DITHIOTHREITOL/10__3390_slash_agronomy13081990-66-72-102
    Average 99 stars, based on 1 article reviews
    sequencing ladder mix - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

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